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α1 –Microglobulin, Highly Purified AJ 3006

Product Details

Data Sheet

PRODUCT CODE

AJ 3006

SHELF-LIFE

5 years from date of manufacture.

SOURCE

Isolated from human urine from patients with chronic renal tubular proteinuria. The raw material urine is tested negative for HBsAg, anti-HIV-1/2 and anti-HCV.

PRESENTATION

Presented freeze-dried from 0.02M ammonium bicarbonate. The product may contain traces of buffer salts. Note: This product is characteristically yellow-brown in colour.

PURITY

Nominally greater than 96% of the protein is α1-microglobulin.

ANALYSIS

Type	Result
SDS-Polyacrylamide Gel Electrophoresis	To show one band corresponding to the molecular weight (27 kD) of α1-M. Under certain conditions the dimer (54 kD) can be observed.
Cellulose Acetate Electrophoresis	To show one band only with α1-electrophoretic mobility.
Total protein concentration by optical density at 280nm using E^280nm_1cm,0.1% = 1.58	>1mg/ml (dispensing solution)
Total protein concentration by Lowry method	To confirm optical density result.
Quantitative and qualitative analysis by Radial Immunodiffusion (RID) using in-house standard	To confirm identity and verify dispensing concentration.

RECONSTITUTION

Use of a phosphate buffer, pH >7.0, containing 0.15M NaCl is recommended.

STORAGE

+2 to +8ºC (do not freeze)

α1 –Microglobulin, Highly Purified AJ 3006