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HIV-1-p24gag, Recombinant AG 6054
Product Details
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Data Sheet |
| PRODUCT CODE | AG 6054 |
| EXPIRY DATE | See lot specific certificate of analysis. |
| PRESENTATION | Freeze-dried in 1mM sodium citrate, pH 5, without preservative. |
| COMPOSITION | Recombinant capsid protein HIV-1-p24 consisting of 234 amino acids. The amino acid sequence is MNSAM-aa(3-231) in comparison to the authentic HIV-1-p24 gag protein (BH 10) published by Ratner et al. (1985), Nature, 313, 277-284 |
| PREPERATION | Direct expression of the capsid protein by use of the translation coupling effect between the starting partial protein lambda cro and the sequence of the capsid protein HIV-p24gag. Host cell E. coli LE392; expression plasmid : pTCA5. Final purification is achieved by FPLC ion-exchange chromatography on Mono S-Sepharose Fast Flow (Pharmacia). |
| ACTIVITY | The serological activity of this recombinant antigen has been checked by a sandwich ELISA using epitope specific anti-p24 (HIV-1) polyclonal antibodies as capture antibodies and a monoclonal anti-p24 (HIV-1) antibody as detection antibody. |
| RECONSTITUTION | Reconstitute with sterile double distilled water. The amount of sterile double distilled water can vary depending on the final concentration desired. |
| STORAGE | +2 to +8°C. Reconstituted product should be stored in aliquots at -20°C. |
| FOR RESEARCH USE ONLY | |
HIV-1-p24gag, Recombinant AG 6054
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