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Product Catalogue

Human NT-proBNP, recombinant BF 3357

Product Details
Download PDF Data Sheet for BF 3357 Data Sheet
PRODUCT CODE BF 3357
SHELF-LIFE 1 year from the date of manufacture
SOURCE Recombinant NT-proBNP expressed in E coli.
An extra N-terminal methionine residue is present
which is not found in the native format.
PRESENTATION Frozen liquid in 150mM sodium chloride, 10mM potassium phosphate, pH 7.4.
PURIFICATION METHOD The protein is purified by ion exchange and affinity chromatography method.
PURITY >95% by SDS-PAGE
MOLECULAR WEIGHT 8.589 kDA confirmed by matrix assisted laser desorption
ionization mass spectrometry.
PROTEIN CONCENTRATION Reported in mg/ml by OD at 280nm (extinction
coefficient 0.814).
DILUTION BUFFER It is recommended to use buffer solutions containing
carrier protein and detergent (e.g. 0.05 M Tris-HCl,
pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine
serum albumin) when diluting samples.
STORAGE -70ºC. Avoid repeated freezing and thawing.
Human NT-proBNP, recombinant BF 3357
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